Vitamin E Assay Kit

Business Type
Manufacturer
Country
China

Quick Details

Brand Name: Elabscience
Model Number: Catalog number:E-BC-K033
Place of Origin: USA
MOQ: 1
Certificate: ISO9001:2008

Product Detail

Catalog number: E-BC-K033 Detection method:Colorimetric method Detection instrument: Spectrophotometry (Visible range) Valid period: 3 months Lead Time: 7~10 days Experimental instrument Glass tube, Micropipettor, Vortex mixer, Centrifuge, Spectrophotometer (533 nm wavelength) Application This kit can be used for detection of VE content in samples, such as animal/plant tissue, serum (plasma), drink, drug, oral liquid, food, etc. Detection significance VE is a kind of natural lipid-soluble antioxidant, which exist in cellular membrane structure (cell membrane, mitochondrial, microsomal membrane), lipid droplets of adipocytes and lipoproteins of plasma. It is scavenger of singlet oxygen and superoxide, blocking agent of lipid peroxidation and can protect protein sulfhydryl. Detection principle Fe3+ can be deoxidized to Fe2+ by VE with ferroin existing.Fe2+ can react with ferroin and form pink compound under certain condition. After colorimetric assay, VE content can be figured out according to the standard curve or calculated through formula. Note: Liquid in the tube will be divided into three layers, and the upper layer is n-heptane VE extraction solution, the middle layer is water and absolute ethyl alcohol and the lower layer is protein precipitate. Detection of VE content in tissue (1) Sample pretreatment: Preparation of 10% homogenate solution: weigh the tissueaccurately. Add Reagent 4 (homogenate medium) in a weight (g): volume (mL) ratio of 1: 9, then use mechanical homogenization in ice water bath. Centrifuge the mixture solution at 2500 rpm for10 min and take the supernatant for measurement. (2) Extraction of n-heptane VE in tissue homogenate (n-heptane is not provided in this kit) Vortex mixing (extract thoroughly) for 1 min, then centrifuge for 5~10 min at 3000~4000 rpm. Take the n-heptane VE extraction solution (the upper layer solution) for chromogenic reaction. Note: Liquid in the tube will be divided into three layers, and the upper layer is n-heptane VE extraction solution, the middle layer is water and absolute ethyl alcohol and the lower layer is protein precipitate. (3)Chromogenic reaction: Mix fully and stand for 2 min. Measure the OD values of each tube at 533 nm wavelength with cuvette of 1cm diameter, set to zero with absolute ethyl alcohol.

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About our Company

Business Type
Manufacturer
Established
2011
Employess
201 - 300 People
Annual Revenue
US$5 Million - US$10 Million
Main Markets
North America, Eastern Europe, East Asia, Africa, South America, Southern Europe, South Asia, Oceania, Central America, Western Europe, Middle East
Products
Laboratory Reagents, Vitamin E

Contact Us

Elabscience Biotechnology,Inc.

Ms. Emily Yang

14780 Memorial Drive, Suite 216.

Houston Texas 77079 China

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