Catalog number: E-CL-H0020
Detection Range: 7.81~500 pg/mL
Sensitivity: 4.69 pg/mL
Lead Time: 7-10 days
Assay type Sandwich
Assay time 4.5h
Detection method: Chemiluminescence
Detection range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample volume 100μL
Sample type Serum, plasma and other biological fluids
Repeatability CV < 15%
This CLIA kit can be applied to the in vitro quantitative determination of Human MCP-1 concentrations in serum, plasma and other biological fluids.
his kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCP-1. The concentration of Human MCP-1 in the samples can be calculated by comparing the RLU of the samples to the standard curve.